Anti-Acetylcholinesterase (human brain, bovine brain, AChE)
Mouse monoclonal antibody
HYB 190-01 is specific for brain AChE and does not recognize AChE from erythrocytes. The antibody can thus distinguish between mammalian brain AChE and erythrocyte AChE.
C-terminal 10 residues of brain acetylcholinesterase (human and bovine), absent from the erythrocyte enzyme.
ELISA, WB, IHC
Species reactivity (positive)
Human, bovine, Torpedo marmorata
Species reactivity (negative)
Electric eel, human
C-terminal 10 residues (aa 574-583) of brain acetylcholinesterase (DS-AChE and SS-AChE) (1).
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
4-8ºC without exposure to light. No precautions necessary during handling.
Acetylcholinesterase (AChE, EC.220.127.116.11.) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320 kDa, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170 kDa.
Detection of higher levels of AChE in amniotic fluid can indicate fetal malformations such as neural tube defects.