DAB, pH 5.0
DAB (3,3-diaminobenzidine tetrahydrochloride), is used as a chromogen substrate in immunoblotting.
DAB produces a brown color during the enzymatic degradation of H2O2 by horseradish peroxidase.
1 tablet dissolved in 40 ml of distilled water will give a solution with pH 5.0 +0.1 and a concentration of 0.25 mg/ml DAB buffered with 20 mM phosphate.
Standard package size is 50 tablets/vial.
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