OPD Tablets for colorimetric detection of Horseradish Peroxidase activity in ELISAs.

Ortho-Phenylenediamine (OPD) is a water soluble chromogenic substrate that produces a yellow color during the enzymatic degradation of hydrogen peroxide by horseradish peroxidase (HRP) with an absorption maximum at 450 nm. After addition of sulfuric acid a very stable orangeend solution is obtained that can be read at 490 nm.

Kem-En-Tec Diagnostics offers:

  • Standard OPD tablets: available with a content of 2 mg (#4090) or 10 mg (#4260)
  • Fizzing OPD tablets: 2 mg tablets are available as fizzing, buffer-containing tablets (#4110)


Catalog number Tablet type Tablet size Package size
4260 Standard OPD tablet 10 mg 100 tablets/vial
4090 Standard OPD tablets 2 mg 250 tablets/vial
4110 Fizzing OPD tablets 2 mg 250 tablets/vial



  • Stable and robust
  • Negligible batch-to-batch variation
  • Development time: 5-30 minutes


  • At least 18 months at 2-8° C
  • For long term storage OPD tablets should be kept at -20°C

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