TMB ONEready-to-use substrate for colorimetric detection in ELISAs based on Horseradish Peroxidase labeled antibodies.

  • Ready-to-use, Mono-component, one-step chromogenic substrate containing 3,3′,5,5′-tetramethylbenzidine, substrate buffer and hydrogen peroxide
  • gives higher signal and has a higher sensitivity in ELISA than OPD (orthophenylene diamine)
  • Negligible batch-to-batch variations



  • Extremely stable and robust
  • Free of harmful organic solvents
  • Development time: 5-30 minutes
  • Stop solution: 0.2 M H2SO4


  • At least 4 years at 2-8° C
  • At least 1 year at room temperature
  • 8 weeks at 37° C

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Memory usage: real: 30932992, emalloc: 30308280
Code ProfilerTimeCntEmallocRealMem