OPD Tablets for colorimetric detection of Horseradish Peroxidase activity in ELISAs.
Ortho-Phenylenediamine (OPD) is a water soluble chromogenic substrate that produces a yellow color during the enzymatic degradation of hydrogen
peroxide by horseradish peroxidase (HRP) with an absorption maximum at 450 nm. After addition of sulfuric acid a very stable orange end solution is
obtained that can be read at 490 nm.
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